This past summer my mentee, Nyrobi Barnes, and I worked on the isolation and characterization of primary mouse microglial cells. We not only worked on the isolation and care of the microglial cells, but we also optimized protocols for detecting the presence of these cells, including Western blots and Immunofluorescence. One of the biggest things we learned this semester was how to problem solves and troubleshoot our experiments.
President’s Office Student Associate
In the King Lab, our research focuses on HIV Associated Neurocognitive Disorders (HAND). We look at how HIV affects the ability of the microglia to do their job and clear debris and dead cells from the brain. Our hypothesis is that the changes in the cell’s ability to undergo phagocytosis is due to abnormalities in the cytoskeleton and its protein levels. To do this we use cell culture, phagocytosis assays, immunofluorescent staining, and Western blots. In the future we hope to replicate the model BV-2 experiments we have already done with isolated primary mouse microglia.
My Research Conferences
We will be attending the Society for Neuroscience Conference in Washington, DC. (https://www.sfn.org/annual-meeting/neuroscience-2017)
Life After Ursinus
After Ursinus I hope to pursue a career in pharmaceutical sales/marketing.