Macrophages aid in the repair of skeletal muscle and are present at the site of injury approximately 48 hours post injury. The mechanism that we focus on is endothelial permeability. This is because without endothelial permeability macrophages, along with various other cells and proteins, cannot reach the injured area and start the healing process. To view permeability, we inject Evans blue dye into the bloodstream, which binds to the protein albumin. When albumin is present there is a change in color in the organ or injured area that is being examined. The purpose of this study is to present a timeline of endothelial permeability 48 hours post skeletal muscle injury, examine differences in female and male endothelial permeability during repair, and find differences in endothelial permeability in mice at ages varying from one month to twelve months, as well as compare endothelial permeability differences between 3 month old ovariectemized females vs unovariectemized females and castrated males vs uncastrated males.
Health and Exercise Physiology