Insulin is a hormone that is critical in the maintenance of blood glucose, which is an important source of energy for all cells. In individuals with type 1 diabetes, glucose homeostasis is impaired because of a lack of sufficient insulin production. The continual reliance on external insulin may disrupt the insulin signaling pathway, which may contribute to the development of insulin resistance. According to studies, exercise training is an effective treatment to regulate insulin resistance in type 1 diabetics. Therefore, the purpose of this study is to examine the effects of exercise training on blood glucose homeostasis and insulin signaling in type 1 diabetic mice. C57BL/6-Ncrl mice will be injected with streptozotocin (STZ), a compound that targets and destroys the beta-cells of the pancreas, to induce type 1 diabetes. After 2 weeks, mice will be exercise trained on a treadmill for 60 minutes a day for 5 days a week for 4 weeks. At the end of 4 weeks of training, mice will be subjected to a glucose tolerance test to assess blood glucose homeostasis. Afterwards, serum will be collected and blood chemistry analysis of insulin and hemoglobin A1c will be conducted. Skeletal muscle and heart will be harvested to analyze genes involved in the insulin signaling pathway. This project will be important in establishing a type 1 diabetic mouse model that can be used by future students in the laboratory, as well as providing a preliminary study in examining the effects of exercise training on glucose metabolism.
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Health and Exercise Physiology